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pgc 1a  (Santa Cruz Biotechnology)


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    Structured Review

    Santa Cruz Biotechnology pgc 1a
    Pgc 1a, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 96/100, based on 1439 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pgc+1a/PGC-1%CE%B1+Antibody/pm41173187-57-17-18
    Average 96 stars, based on 1439 article reviews
    pgc 1a - by Bioz Stars, 2026-09
    96/100 stars

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    Related Articles

    Nucleic Acid Electrophoresis:

    Article Title: The Synergistic Cytotoxic Effects of GW5074 and Sorafenib by Impacting Mitochondrial Functions in Human Colorectal Cancer Cell Lines.
    Article Snippet: .. Proteins in the resultant lysates were separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and analyzed by immunoblotting with antibodies against ACTN (sc-17829, mouse), ATF1 (sc-243, mouse), ATF3 (sc-81189, mouse), ATF4 (sc-390063, mouse), ATF5 (sc-377168, mouse), catalase (sc-271803, mouse), DRP-1 (sc-101270, mouse), Mfn1 (sc-166644, mouse), Nrf2 (sc-365949, mouse), p62 (sc-28359, mouse), Parkin (sc-133167, mouse), PGC-1a (sc-518052, mouse), SOD1 (sc-101523, mouse), SOD2 (sc-133134, mouse), SOD3 (sc-377168, mouse), mtTFA (sc376672, mouse), TFEB (sc-166736, mouse), Tom20 (sc-17764, mouse) (Santa Cruz Biotechnology, CA, USA), HO-1 (ADI-SPA895-F, rabbit, Enzo life sciences, NY, USA), g.H2AX (ab81299, rabbit, Abcam, Cambridge, UK), GAPDH (60004-1-1g, mouse), a-Tubulin (13730-1-AP, mouse) (Proteintech, IL, USA), XBP1 (NBP1-77253, rabbit), ATF6 (NBP1-40256, mouse) (Novus, CO, USA), AMPK (2535, rabbit), p-AMPK (5831, rabbit), Caspase 3 (9662, rabbit), CHOP (2895, mouse), p70S6K (2708, rabbit), pp70S6K (9205, rabbit). p-DRP-1 (3455, rabbit), eIF2a (9722, rabbit), p-eIF2a (9721, rabbit), LC3B (2775, rabbit), and PARP (9542, rabbit) (Cell Signaling Technology, MA, USA). .. Thereafter, the blots were incubated with horseradish peroxidase-conjugated secondary antibody (Santa Cruz Biotechnology).

    Western Blot:

    Article Title: The Synergistic Cytotoxic Effects of GW5074 and Sorafenib by Impacting Mitochondrial Functions in Human Colorectal Cancer Cell Lines.
    Article Snippet: .. Proteins in the resultant lysates were separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and analyzed by immunoblotting with antibodies against ACTN (sc-17829, mouse), ATF1 (sc-243, mouse), ATF3 (sc-81189, mouse), ATF4 (sc-390063, mouse), ATF5 (sc-377168, mouse), catalase (sc-271803, mouse), DRP-1 (sc-101270, mouse), Mfn1 (sc-166644, mouse), Nrf2 (sc-365949, mouse), p62 (sc-28359, mouse), Parkin (sc-133167, mouse), PGC-1a (sc-518052, mouse), SOD1 (sc-101523, mouse), SOD2 (sc-133134, mouse), SOD3 (sc-377168, mouse), mtTFA (sc376672, mouse), TFEB (sc-166736, mouse), Tom20 (sc-17764, mouse) (Santa Cruz Biotechnology, CA, USA), HO-1 (ADI-SPA895-F, rabbit, Enzo life sciences, NY, USA), g.H2AX (ab81299, rabbit, Abcam, Cambridge, UK), GAPDH (60004-1-1g, mouse), a-Tubulin (13730-1-AP, mouse) (Proteintech, IL, USA), XBP1 (NBP1-77253, rabbit), ATF6 (NBP1-40256, mouse) (Novus, CO, USA), AMPK (2535, rabbit), p-AMPK (5831, rabbit), Caspase 3 (9662, rabbit), CHOP (2895, mouse), p70S6K (2708, rabbit), pp70S6K (9205, rabbit). p-DRP-1 (3455, rabbit), eIF2a (9722, rabbit), p-eIF2a (9721, rabbit), LC3B (2775, rabbit), and PARP (9542, rabbit) (Cell Signaling Technology, MA, USA). .. Thereafter, the blots were incubated with horseradish peroxidase-conjugated secondary antibody (Santa Cruz Biotechnology).

    Article Title: Adaptive gene expression of alternative splicing variants of PGC-1α regulates whole-body energy metabolism.
    Article Snippet: Immunoblot analysis was performed with antibodies to PGC-1a (ST1202, Calbiochem), to mitochondrial complex II (459200, Invitrogen), and to mitochondrial complex III (459140, Invitrogen), as well as with those to glyceraldehyde-3-phosphate dehydrogenase (GAPDH) as a loading control (M171-7, MBL). .. COS7 cells transfected with pcDNA3.1-based expression vectors encoding mouse PGC-1a isoforms and mouse PPARa were subjected to immunoprecipitation with antibodies to PGC-1a (ST1202, Calbiochem), and the resulting precipitates were subjected to immunoblot analysis with the same antibodies to PGC-1a and with those to PPARa (H-08, Santa Cruz Biotechnology). ..

    Article Title: FGF21-FGFR1 signaling protects against cardiac hypertrophy by regulating PINK1-mediated mitophagy pathway.
    Article Snippet: .. The following commercial antibodies were used: FGF21 (abclonal, A3908), ANP (santa cruz, sc-515701), BNP (santa cruz, sc271185), PGC-1a (santa cruz, sc-518025), Mfn2 (proteintech, 12186–1-AP), Drp1 (abcam, Ab56788), PINK1 rabbit mAb (abcam, Ab75487), PARKIN rabbit mAb (santa cruz, sc-32282), SQSTM1/ P62 (santa cruz, sc-48402), LC3A/B (abcam, Ab128025), Beclin1 (abcam, Ab114071), VDAC (abcam, Ab15895), FUNDC1 (CST, E2F4T), BNIP3L/NIX (CST, 12396S), GAPDH (sangon, D110016); total OXPHOS WB antibody cocktail (abcam, ab110413). .. Phenylephrine (PE, #HY-B0769) and FGF21 protein(#HY-P70473), Efruxifermin (#HY-P99930) and rapamycin (Rapa, #HY-10219) were purchased from MCE.

    Pyrolysis Gas Chromatography:

    Article Title: The Synergistic Cytotoxic Effects of GW5074 and Sorafenib by Impacting Mitochondrial Functions in Human Colorectal Cancer Cell Lines.
    Article Snippet: .. Proteins in the resultant lysates were separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and analyzed by immunoblotting with antibodies against ACTN (sc-17829, mouse), ATF1 (sc-243, mouse), ATF3 (sc-81189, mouse), ATF4 (sc-390063, mouse), ATF5 (sc-377168, mouse), catalase (sc-271803, mouse), DRP-1 (sc-101270, mouse), Mfn1 (sc-166644, mouse), Nrf2 (sc-365949, mouse), p62 (sc-28359, mouse), Parkin (sc-133167, mouse), PGC-1a (sc-518052, mouse), SOD1 (sc-101523, mouse), SOD2 (sc-133134, mouse), SOD3 (sc-377168, mouse), mtTFA (sc376672, mouse), TFEB (sc-166736, mouse), Tom20 (sc-17764, mouse) (Santa Cruz Biotechnology, CA, USA), HO-1 (ADI-SPA895-F, rabbit, Enzo life sciences, NY, USA), g.H2AX (ab81299, rabbit, Abcam, Cambridge, UK), GAPDH (60004-1-1g, mouse), a-Tubulin (13730-1-AP, mouse) (Proteintech, IL, USA), XBP1 (NBP1-77253, rabbit), ATF6 (NBP1-40256, mouse) (Novus, CO, USA), AMPK (2535, rabbit), p-AMPK (5831, rabbit), Caspase 3 (9662, rabbit), CHOP (2895, mouse), p70S6K (2708, rabbit), pp70S6K (9205, rabbit). p-DRP-1 (3455, rabbit), eIF2a (9722, rabbit), p-eIF2a (9721, rabbit), LC3B (2775, rabbit), and PARP (9542, rabbit) (Cell Signaling Technology, MA, USA). .. Thereafter, the blots were incubated with horseradish peroxidase-conjugated secondary antibody (Santa Cruz Biotechnology).

    Article Title: Honokiol alleviated neurodegeneration by reducing oxidative stress and improving mitochondrial function in mutant SOD1 cellular and mouse models of amyotrophic lateral sclerosis.
    Article Snippet: .. Membranes were blocked by the 5% skimmed milk solution for 2 h at room temperature and then incubated at 4 C overnight with antibodies against BCL2 (26539- 1-AP, 1:1000), BAX (50599-2-Ig, 1:2000), cytochrome c (10993- 1-AP, 1:2000), ATM (27156-1-AP, 1:1000), NRF2 (16396-1-AP, 1:1000), GCLC (12601-1-AP, 1:2000), GCLM (14241-1-AP, 1:2000), OPA1 (27733-1-AP, 1:1000), MFN2 (12186-1-AP, 1:2000), FIS1 (10956-1-AP, 1:1000), p62 (18420-1-AP, 1:1000), GFAP (26825-1-AP, 1:2000), HSP90 (13171-1-AP, 1:2000), GAPDH (60004-1-Ig, 1:5000) from Proteintech (Rosemont, IL, USA); GSR (sc-133245, 1:500), GSS (sc-166882, 1:500), VDAC1 (sc-390996, 1:500), PGC-1a (sc-518025, 1:500), NRF1 (sc515360, 1:500), TFAM (sc-166965, 1:500) from Santa Cruz Biotechnology (Dallas, TX, USA); PINK1 (BC100-494S, 1:500) from Novus biological (Littleton, CO, USA); 4-HNE (ab46545, 1:1000), HO-1 (ab68477, 1:2000), NQO1 (ab80588, 1:1000) from Abcam (Cambridge, UK). .. Phospho-Histone H2AX (Ser139) (2577S, 1:1000), Lamin A/C (4777S, 1:1000) from Cell Signaling Technology (Danvers, MA, USA).

    Article Title: Adaptive gene expression of alternative splicing variants of PGC-1α regulates whole-body energy metabolism.
    Article Snippet: Immunoblot analysis was performed with antibodies to PGC-1a (ST1202, Calbiochem), to mitochondrial complex II (459200, Invitrogen), and to mitochondrial complex III (459140, Invitrogen), as well as with those to glyceraldehyde-3-phosphate dehydrogenase (GAPDH) as a loading control (M171-7, MBL). .. COS7 cells transfected with pcDNA3.1-based expression vectors encoding mouse PGC-1a isoforms and mouse PPARa were subjected to immunoprecipitation with antibodies to PGC-1a (ST1202, Calbiochem), and the resulting precipitates were subjected to immunoblot analysis with the same antibodies to PGC-1a and with those to PPARa (H-08, Santa Cruz Biotechnology). ..

    Article Title: RNA-binding protein YBX3 promotes PPARγ-SLC3A2 mediated BCAA metabolism fueling brown adipogenesis and thermogenesis.
    Article Snippet: Objective: Activating brown adipose tissue (BAT) thermogenesis is a promising approach to combat obesity and metabolic disorders.. The posttranscriptional regulation of BAT thermogenesis mediated by RNA-binding proteins (RBPs) is still not fully understood.. This study explores the physiological role of novel RBPs in BAT differentiation and thermogenesis.

    Article Title: FGF21-FGFR1 signaling protects against cardiac hypertrophy by regulating PINK1-mediated mitophagy pathway.
    Article Snippet: .. The following commercial antibodies were used: FGF21 (abclonal, A3908), ANP (santa cruz, sc-515701), BNP (santa cruz, sc271185), PGC-1a (santa cruz, sc-518025), Mfn2 (proteintech, 12186–1-AP), Drp1 (abcam, Ab56788), PINK1 rabbit mAb (abcam, Ab75487), PARKIN rabbit mAb (santa cruz, sc-32282), SQSTM1/ P62 (santa cruz, sc-48402), LC3A/B (abcam, Ab128025), Beclin1 (abcam, Ab114071), VDAC (abcam, Ab15895), FUNDC1 (CST, E2F4T), BNIP3L/NIX (CST, 12396S), GAPDH (sangon, D110016); total OXPHOS WB antibody cocktail (abcam, ab110413). .. Phenylephrine (PE, #HY-B0769) and FGF21 protein(#HY-P70473), Efruxifermin (#HY-P99930) and rapamycin (Rapa, #HY-10219) were purchased from MCE.

    Article Title: Expansion of T memory stem cells with superior anti-tumor immunity by Urolithin A-induced mitophagy.
    Article Snippet: .. Then, tumor immune filtrates or activated T cells were stained with Fixable Viability Dye-eF780 (65-0865-14; eBioscience), aCD45-FITC (30-F11; 11-0451-85; eBioscience), aCD11bBV650 (M1/70; 56340; BD) a-CD11c-BV785 (HL3; 563735; BD), aLy6G-BV605 (53-6.7; 563005; BD), aLy6C-AF700 (HK1.4; 128024; Biolegend), aF4/80-PE (BM8; 12-4801-82, eBioscience), aPD-L1-PECy7 (MIH5; 25-5982-80, eBioscience); or with Fixable Viability Dye-eF780 (ebioscience), aCD45-BV786 (30-F11; 564225; BD), aCD4-BUV496 (GK1.5; 564667; BD), aCD8-BV605 (100744; Biolegend), aPD1-APC (J43; 17-9985-82; eBioscience), aTIM-3-PECy7 (RMT3-23; 119715; Biolegend), aCTLA4-PE (UC10-4B9; 106305; Biolegend) and aLag3-BV421 (C9B7W; 125221, Biolegend); or with Fixable Viability Dye-eF780 (eBioscience), aCD4FITC (H129.19; 553650; BD), aCD8-APC (53-6.7; 17-0081-83; eBioscience), aCD44-AF400 (IM7; 103026; Biolegend), aCD62LBV785 (MEL-14; 104440; Biolegend), aSca-1-PerCP-Cy5.5 (D7; 45-5981-82; eBioscience), aCD95-PE (Jo2; 554258; BD); or with Fixable Viability Dye-eF780 (eBioscience), aCD8-BV650 (53-6.7; 563234; BD), aCD4-BUV496 (GK1.5; 564667; BD), aCD127-APC (A7R34; 17-1271-82; eBioscience), aCD95-FITC (SA367H8;152605; Biolegend), aCD44-AF400 (IM7; 103026; Biolegend), aCD62L-BV785 (MEL-14; 104440; Biolegend), aSca-1-PerCP-Cy5.5 (D7; 45-5981-82; eBioscience) followed by intracellular staining of aTCF1-PE (S33-966; 564217; BD), or PGC-1a (sc-518025; D-5; santa cruz), or aCyclinD1 (MA5-28534, DCS-6; Invitrogen) with transcription factor buffer set kit (BD Biosciences). .. To study cytokine expression, tumor immune infiltrates were stimulated ex vivo with 20ng/ml PMA and 1mg/ml Ionomycin (Sigma) for 3h in T cell activation medium with Brefeldin A (Biolegend).

    Article Title: Bone marrow immune cells respond to fluctuating nutritional stress to constrain weight regain.
    Article Snippet: Article Bone marrow immune cell s respond to fluctuating nutritional stress to constrain weight regain

    Incubation:

    Article Title: Honokiol alleviated neurodegeneration by reducing oxidative stress and improving mitochondrial function in mutant SOD1 cellular and mouse models of amyotrophic lateral sclerosis.
    Article Snippet: .. Membranes were blocked by the 5% skimmed milk solution for 2 h at room temperature and then incubated at 4 C overnight with antibodies against BCL2 (26539- 1-AP, 1:1000), BAX (50599-2-Ig, 1:2000), cytochrome c (10993- 1-AP, 1:2000), ATM (27156-1-AP, 1:1000), NRF2 (16396-1-AP, 1:1000), GCLC (12601-1-AP, 1:2000), GCLM (14241-1-AP, 1:2000), OPA1 (27733-1-AP, 1:1000), MFN2 (12186-1-AP, 1:2000), FIS1 (10956-1-AP, 1:1000), p62 (18420-1-AP, 1:1000), GFAP (26825-1-AP, 1:2000), HSP90 (13171-1-AP, 1:2000), GAPDH (60004-1-Ig, 1:5000) from Proteintech (Rosemont, IL, USA); GSR (sc-133245, 1:500), GSS (sc-166882, 1:500), VDAC1 (sc-390996, 1:500), PGC-1a (sc-518025, 1:500), NRF1 (sc515360, 1:500), TFAM (sc-166965, 1:500) from Santa Cruz Biotechnology (Dallas, TX, USA); PINK1 (BC100-494S, 1:500) from Novus biological (Littleton, CO, USA); 4-HNE (ab46545, 1:1000), HO-1 (ab68477, 1:2000), NQO1 (ab80588, 1:1000) from Abcam (Cambridge, UK). .. Phospho-Histone H2AX (Ser139) (2577S, 1:1000), Lamin A/C (4777S, 1:1000) from Cell Signaling Technology (Danvers, MA, USA).

    Article Title: Bone marrow immune cells respond to fluctuating nutritional stress to constrain weight regain.
    Article Snippet: Article Bone marrow immune cell s respond to fluctuating nutritional stress to constrain weight regain

    Transfection:

    Article Title: Adaptive gene expression of alternative splicing variants of PGC-1α regulates whole-body energy metabolism.
    Article Snippet: Immunoblot analysis was performed with antibodies to PGC-1a (ST1202, Calbiochem), to mitochondrial complex II (459200, Invitrogen), and to mitochondrial complex III (459140, Invitrogen), as well as with those to glyceraldehyde-3-phosphate dehydrogenase (GAPDH) as a loading control (M171-7, MBL). .. COS7 cells transfected with pcDNA3.1-based expression vectors encoding mouse PGC-1a isoforms and mouse PPARa were subjected to immunoprecipitation with antibodies to PGC-1a (ST1202, Calbiochem), and the resulting precipitates were subjected to immunoblot analysis with the same antibodies to PGC-1a and with those to PPARa (H-08, Santa Cruz Biotechnology). ..

    Expressing:

    Article Title: Adaptive gene expression of alternative splicing variants of PGC-1α regulates whole-body energy metabolism.
    Article Snippet: Immunoblot analysis was performed with antibodies to PGC-1a (ST1202, Calbiochem), to mitochondrial complex II (459200, Invitrogen), and to mitochondrial complex III (459140, Invitrogen), as well as with those to glyceraldehyde-3-phosphate dehydrogenase (GAPDH) as a loading control (M171-7, MBL). .. COS7 cells transfected with pcDNA3.1-based expression vectors encoding mouse PGC-1a isoforms and mouse PPARa were subjected to immunoprecipitation with antibodies to PGC-1a (ST1202, Calbiochem), and the resulting precipitates were subjected to immunoblot analysis with the same antibodies to PGC-1a and with those to PPARa (H-08, Santa Cruz Biotechnology). ..

    Immunoprecipitation:

    Article Title: Adaptive gene expression of alternative splicing variants of PGC-1α regulates whole-body energy metabolism.
    Article Snippet: Immunoblot analysis was performed with antibodies to PGC-1a (ST1202, Calbiochem), to mitochondrial complex II (459200, Invitrogen), and to mitochondrial complex III (459140, Invitrogen), as well as with those to glyceraldehyde-3-phosphate dehydrogenase (GAPDH) as a loading control (M171-7, MBL). .. COS7 cells transfected with pcDNA3.1-based expression vectors encoding mouse PGC-1a isoforms and mouse PPARa were subjected to immunoprecipitation with antibodies to PGC-1a (ST1202, Calbiochem), and the resulting precipitates were subjected to immunoblot analysis with the same antibodies to PGC-1a and with those to PPARa (H-08, Santa Cruz Biotechnology). ..

    Aqueous Normal-phase Chromatography:

    Article Title: FGF21-FGFR1 signaling protects against cardiac hypertrophy by regulating PINK1-mediated mitophagy pathway.
    Article Snippet: .. The following commercial antibodies were used: FGF21 (abclonal, A3908), ANP (santa cruz, sc-515701), BNP (santa cruz, sc271185), PGC-1a (santa cruz, sc-518025), Mfn2 (proteintech, 12186–1-AP), Drp1 (abcam, Ab56788), PINK1 rabbit mAb (abcam, Ab75487), PARKIN rabbit mAb (santa cruz, sc-32282), SQSTM1/ P62 (santa cruz, sc-48402), LC3A/B (abcam, Ab128025), Beclin1 (abcam, Ab114071), VDAC (abcam, Ab15895), FUNDC1 (CST, E2F4T), BNIP3L/NIX (CST, 12396S), GAPDH (sangon, D110016); total OXPHOS WB antibody cocktail (abcam, ab110413). .. Phenylephrine (PE, #HY-B0769) and FGF21 protein(#HY-P70473), Efruxifermin (#HY-P99930) and rapamycin (Rapa, #HY-10219) were purchased from MCE.

    Staining:

    Article Title: Expansion of T memory stem cells with superior anti-tumor immunity by Urolithin A-induced mitophagy.
    Article Snippet: .. Then, tumor immune filtrates or activated T cells were stained with Fixable Viability Dye-eF780 (65-0865-14; eBioscience), aCD45-FITC (30-F11; 11-0451-85; eBioscience), aCD11bBV650 (M1/70; 56340; BD) a-CD11c-BV785 (HL3; 563735; BD), aLy6G-BV605 (53-6.7; 563005; BD), aLy6C-AF700 (HK1.4; 128024; Biolegend), aF4/80-PE (BM8; 12-4801-82, eBioscience), aPD-L1-PECy7 (MIH5; 25-5982-80, eBioscience); or with Fixable Viability Dye-eF780 (ebioscience), aCD45-BV786 (30-F11; 564225; BD), aCD4-BUV496 (GK1.5; 564667; BD), aCD8-BV605 (100744; Biolegend), aPD1-APC (J43; 17-9985-82; eBioscience), aTIM-3-PECy7 (RMT3-23; 119715; Biolegend), aCTLA4-PE (UC10-4B9; 106305; Biolegend) and aLag3-BV421 (C9B7W; 125221, Biolegend); or with Fixable Viability Dye-eF780 (eBioscience), aCD4FITC (H129.19; 553650; BD), aCD8-APC (53-6.7; 17-0081-83; eBioscience), aCD44-AF400 (IM7; 103026; Biolegend), aCD62LBV785 (MEL-14; 104440; Biolegend), aSca-1-PerCP-Cy5.5 (D7; 45-5981-82; eBioscience), aCD95-PE (Jo2; 554258; BD); or with Fixable Viability Dye-eF780 (eBioscience), aCD8-BV650 (53-6.7; 563234; BD), aCD4-BUV496 (GK1.5; 564667; BD), aCD127-APC (A7R34; 17-1271-82; eBioscience), aCD95-FITC (SA367H8;152605; Biolegend), aCD44-AF400 (IM7; 103026; Biolegend), aCD62L-BV785 (MEL-14; 104440; Biolegend), aSca-1-PerCP-Cy5.5 (D7; 45-5981-82; eBioscience) followed by intracellular staining of aTCF1-PE (S33-966; 564217; BD), or PGC-1a (sc-518025; D-5; santa cruz), or aCyclinD1 (MA5-28534, DCS-6; Invitrogen) with transcription factor buffer set kit (BD Biosciences). .. To study cytokine expression, tumor immune infiltrates were stimulated ex vivo with 20ng/ml PMA and 1mg/ml Ionomycin (Sigma) for 3h in T cell activation medium with Brefeldin A (Biolegend).



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